Technician B

Responsibilities

• Conduct research to understand the pathophysiology of pediatric eosinophilic esophagitis (EoE) and identify novel biomarkers and drug targets.
• Formulate research hypotheses, design and conduct experiments, and implement statistical analyses.
• Maintain mouse lines, including colony maintenance, genotyping, mating, and administering treatments.
• Perform dissections to isolate tissue-specific protein and mRNA for RT-PCR and western immunoblotting.
• Culture 2D and 3D cell systems using mouse cell lines and primary cells.
• Manage laboratory organization, including inventory of supplies, preparation of stock reagents, and management of expenditures.
• Complete all necessary data documentation, coding, cleaning, and entry.
• Participate in weekly work meetings and collaborate with the PI and research team.

Requirements

• Bachelor’s degree in chemistry, biology, or physics, or completion of an animal husbandry program.
• Equivalent combination of education, training, and experience.
• Knowledge of standard laboratory procedures.

Preferred Qualifications

• One year of related research experience.
• Experience in stem cell and epithelial cell biology.
• Experience in human development and disease research.
• Experience conducting independent research experiments and projects.

Benefits

• Comprehensive health and welfare programs.
• Employee assistance programs.
• Tuition assistance programs.
• Retirement benefits.

About the Company

Columbia University Medical Center is a leader in teaching, research, and patient care. The Allergy & Immunology and Gastroenterology Eosinophilic Gastrointestinal Diseases Program conducts advanced research using mouse genetics, 3D organoid culture systems, and human pluripotent stem cells to identify pathways for treating rare and common allergic and immunologic diseases.