Senior Associate Scientist II

About the Company

Prime Medicine is a leading biotechnology company dedicated to creating and delivering the next generation of gene editing therapies to patients. The Company is leveraging its proprietary Prime Editing platform to develop a new class of differentiated, one-time, potentially curative genetic therapies.

Responsibilities

• Manage database and serve as primary liaison for mRNA requests across research and program teams
• Execute mRNA production at various scales, from high-throughput plate-based to GLP toxicology batches
• Provide technical oversight for GMP manufacturing as Person in Plant
• Collaborate with Analytical and QC teams to align testing and release workflows
• Conduct statistical and advanced analytics for process and product knowledge
• Perform in vitro transcription (IVT), capping, and purification operations using AKTA-based chromatography
• Apply DOE principles to optimize reaction conditions
• Operate and train peers on automated systems such as Lynx, Big Tuna, ResolveX, Tecan, and Hamilton

Requirements

• Bachelor’s Degree in Biochemistry, Chemistry, Chemical Engineering, Biomedical Engineering, or related field
• 5 years of experience in drug substance process development
• 4 years of experience with enzymatic reaction process development for mRNA production using DoE
• 5 years of experience in downstream processing with emphasis on chromatography of biologics and RNA
• 3 years of experience with high throughput/automation development and execution
• 4 years of experience with analytical characterization techniques (HPLC/UPLC, TEM, DSC, UV-Vis, ELISA)
• 2 years of experience generating manufacturing batch documents and technical reports for IND/CTA submissions
• 1 year of experience with synthesis and purification of long guide oligonucleotide RNA molecules