Scientist/Senior Scientist, Assay Development

About the Company

At Glyphic Biotechnologies, we plan to create the protein revolution for which scientists and researchers have been waiting. We are developing a massively parallel, single-molecule proteome sequencing platform that will transform life science discovery and usher in a new era of insights into human biology and disease.

Responsibilities

• Design, test, and optimize inputs for ProSE experiments for robustness, sensitivity, and scalability
• Lead the integration of novel chemistry developments into sample preparation and assay workflows
• Optimize and integrate C-terminal and side-chain blocking chemistries into automated assay workflows
• Propose and qualify in-process controls and analytical tools to understand sequencing efficiency
• Apply chemoenzymatic synthesis approaches and characterize individual enzymatic reaction steps
• Introduce and validate real biological sample inputs across a range of sample types and complexities
• Develop extraction and purification protocols for inputs derived from cells, tissues, and blood
• Develop and implement strategies for sample enrichment, depletion, and dynamic range management
• Support the generation and sourcing of standard reference materials using cell culture techniques

Requirements

• Degree in biochemistry, chemical biology, molecular biology, or an equivalent field
• PhD with 4+ years, MS with 6+ years, or BS with 8+ years of relevant experience for Senior Scientist
• PhD with 0+ years, MS with 2+ years, or BS with 4+ years of relevant experience for Scientist I
• Expertise in developing and optimizing ultrasensitive proteomic assays from real biological samples
• Experience with LCMS for discovery proteomics
• Experience integrating chemical labeling or modification chemistries into sample preparation
• Experience developing sample preparation or enrichment methods for low-abundance targets
• Understanding of peptide chemistry and post-translational modification (PTM) preservation

Preferred Qualifications

• Experience processing biological samples from cell lines, blood, or tissue
• Familiarity with highly multiplexed protein quantification platforms (e.g., nELISA, NuLISA, PEA)
• Experience with nanopore platforms for sequencing or label-free detection
• Experience with single-cell or spatial proteomics and transcriptomics
• Proficiency with R or Python for data analysis workflows

Benefits

• Employee Stock Option Plan
• 100% Health Plan Coverage for Employees & Dependents (Medical, Dental, & Vision)
• Employer Retirement Contributions to 401(k)
• Generous Paid Time Off
• Paid Maternity and Paternity Leave
• Health & Wellbeing Program
• Office Snacks and Beverages
• Regular Team Bonding Activities