Principal/Senior Scientist, mRNA engineering

About the Company

NewLimit is a biotechnology company working to radically extend human healthspan. We’re developing medicines to treat age-related diseases by reprogramming the epigenome, a new therapeutic mechanism to restore regenerative potential in aged and diseased cells.

Responsibilities

• Execute and scale end-to-end mRNA production, including template preparation, in vitro transcription (IVT), and purification (TFF, chromatography)
• Implement rigorous quality control assays, including direct RNA sequencing, fragment analysis, dsRNA quantitation, and residual contaminant analysis
• Author and maintain detailed SOPs and batch records, ensuring all production runs meet GLP standards
• Independently design and execute experiments to improve mRNA sequence potency, specificity, and manufacturing performance
• Train, manage, and mentor other team members on mRNA optimization and production workflows

Requirements

• PhD in molecular biology, biochemistry, cell biology, or a related field or equivalent industry experience (5+ years)
• Deep hands-on experience with IVT and the specific challenges of scaling nucleic acid production
• Proficiency with TFF and chromatography systems for the purification of large scale RNA batches
• Ability to perform and interpret mRNA QC assays and troubleshoot mRNA production challenges
• Strong experience in drafting technical reports and standardized protocols

Preferred Qualifications

• Familiarity with Python or R for sequence analysis and secondary structure prediction
• Understanding of how mRNA quality attributes impact Lipid Nanoparticle (LNP) formulation and delivery
• Experience with complex plasmid design and bacterial scale-up for DNA template generation
• Experience managing external contract manufacturing organizations (CMOs)

Benefits

• Health, dental, and vision insurance
• 401(k) with company match
• Paid parental leave
• Industry-leading paid time off (PTO) — 20 days/year for all employees + holidays
• Meals and snacks on-site