Postdoctoral Fellow/Scientist I, Single-Cell Method Development

Responsibilities

• Design, optimize, and scale high-throughput single-cell/nuclei extraction, barcoding, and library preparation workflows for scGRO-seq
• Combine scGRO-seq with CRISPR-based enhancer perturbation, drug treatments, and multi-omics profiling
• Execute advanced molecular biology methods including assay design, cell manipulation, RNA/DNA isolation, PCR/qPCR, and NGS library construction
• Adapt and validate protocols in patient biopsies and 3D organoid models
• Isolate, culture, and reprogram hematopoietic stem/progenitor cells (HSPCs) and primary immune subsets
• Partner with computational biologists to process and interpret large-scale single-cell datasets
• Contribute to publications, preprints, conference presentations, and grant proposals

Requirements

• PhD in Biomedical Engineering, Molecular Biology, Systems Biology, Genetics, or related field
• 4+ years of experience in genomics assay development or method optimization
• 2+ years of experience in single-cell transcriptomics, droplet chemistry, and microfluidics
• Proven expertise in bulk or single-cell method development
• Experience designing and executing CRISPR screens and perturbation assays
• Strong problem-solving and troubleshooting skills
• Excellent communication and collaborative skills

Preferred Qualifications

• Knowledge of microfluidic-based single-cell methods
• Experience with hydrogel synthesis and single-cell barcoded bead generation
• Experience with functional genomics in immune cells
• Experience integrating multi-modal single-cell datasets (RNA, ATAC, proteomics)
• Working knowledge of Linux/HPC environments and tools like Seurat or Scanpy

About the Company

Chan Zuckerberg Biohub New York is an independent nonprofit research institute that brings together Columbia University, The Rockefeller University, and Yale University to investigate the fundamental mechanisms underlying disease and develop new technologies for diagnostics and therapies.