Molecular Biology Scientist/Senior Associate Scientist

Responsibilities

• Execute viral vector plasmid and helper plasmid design and construction
• Perform routine molecular cloning workflows and ensure plasmid quality
• Maintain accurate plasmid records including sequence files and maps
• Generate and characterize engineered mammalian cell lines
• Perform mammalian cell culture and transient transfection under BSL-2 conditions
• Support small-scale vector production using suspension cell lines
• Execute molecular/cellular assays using qPCR/dPCR, ELISA/Western Blot, and flow cytometry
• Analyze data, summarize results, and present conclusions to project leads
• Adhere to BSL-2 biosafety standards and maintain electronic lab notebooks
• Collaborate on tech transfer activities and plan day-to-day execution

Requirements

• M.S. with 5+ years or Ph.D. with 0-2 years in a related field
• Hands-on molecular cloning, cell engineering, and mammalian cell culture
• Experience with plasmid prep, sequence analysis, and construct verification
• Experience generating engineered cell lines (stable expression or CRISPR KO/KI)
• Exposure to viral vector production workflows (LVV preferred)
• Proficiency with qPCR/dPCR, flow cytometry, Western blot, and ELISA
• Experience with CRISPR/Cas gene editing
• Strong ELN documentation and sample tracking skills
• Ability to work in a fast-paced, collaborative environment

Preferred Qualifications

• Experience with viral vector process development (upstream or downstream)
• Exposure to NGS or Nanopore sequencing
• Tech transfer and/or CMC documentation experience

About the Company

Legend Biotech is a global biotechnology company dedicated to treating, and one day curing, life-threatening diseases. We develop advanced cell therapies across diverse technology platforms, including autologous and allogenic CAR-T, TCR-T, and NK cell-based immunotherapy.